Deinococcales
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Deinococcales
''Deinococcota'' (synonym, ''Deinococcus-Thermus'') is a phylum of bacteria with a single class, ''Deinococci'', that are highly resistant to environmental hazards, also known as extremophiles. These bacteria have thick cell walls that give them gram-positive stains, but they include a second membrane and so are closer in structure to those of gram-negative bacteria. Taxonomy The phylum ''Deinococcota'' consists of a single class ('' Deinococci'') and two orders: * The '' Deinococcales'' include two families ('' Deinococcaceae'' and'' Trueperaceae''), with three genera, '' Deinococcus'', '' Deinobacterium'' and '' Truepera''.Garrity GM, Holt JG. (2001) Phylum BIV. "Deinococcus–Thermus". In: Bergey’s manual of systematic bacteriology, pp. 395-420. Eds D. R. Boone, R. W. Castenholz. Springer-: New York.Garrity GM, Bell JA, Lilburn TG. (2005) Phylum BIV. The revised road map to the Manual. In: Bergey’s manual of systematic bacteriology, pp. 159-220. Eds Brenner DJ, Krieg NR, ...
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Deinococcales
''Deinococcota'' (synonym, ''Deinococcus-Thermus'') is a phylum of bacteria with a single class, ''Deinococci'', that are highly resistant to environmental hazards, also known as extremophiles. These bacteria have thick cell walls that give them gram-positive stains, but they include a second membrane and so are closer in structure to those of gram-negative bacteria. Taxonomy The phylum ''Deinococcota'' consists of a single class ('' Deinococci'') and two orders: * The '' Deinococcales'' include two families ('' Deinococcaceae'' and'' Trueperaceae''), with three genera, '' Deinococcus'', '' Deinobacterium'' and '' Truepera''.Garrity GM, Holt JG. (2001) Phylum BIV. "Deinococcus–Thermus". In: Bergey’s manual of systematic bacteriology, pp. 395-420. Eds D. R. Boone, R. W. Castenholz. Springer-: New York.Garrity GM, Bell JA, Lilburn TG. (2005) Phylum BIV. The revised road map to the Manual. In: Bergey’s manual of systematic bacteriology, pp. 159-220. Eds Brenner DJ, Krieg NR, ...
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Deinococci
''Deinococcota'' (synonym, ''Deinococcus-Thermus'') is a phylum of bacteria with a single class, ''Deinococci'', that are highly resistant to environmental hazards, also known as extremophiles. These bacteria have thick cell walls that give them gram-positive stains, but they include a second membrane and so are closer in structure to those of gram-negative bacteria. Taxonomy The phylum ''Deinococcota'' consists of a single class ('' Deinococci'') and two orders: * The ''Deinococcales'' include two families ('' Deinococcaceae'' and'' Trueperaceae''), with three genera, '' Deinococcus'', '' Deinobacterium'' and '' Truepera''.Garrity GM, Holt JG. (2001) Phylum BIV. "Deinococcus–Thermus". In: Bergey’s manual of systematic bacteriology, pp. 395-420. Eds D. R. Boone, R. W. Castenholz. Springer-: New York.Garrity GM, Bell JA, Lilburn TG. (2005) Phylum BIV. The revised road map to the Manual. In: Bergey’s manual of systematic bacteriology, pp. 159-220. Eds Brenner DJ, Krieg NR, S ...
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Deinococcaceae
''Deinococcus'' (from the el, δεινός, ''deinos'', "dreadful, strange" and κόκκος, ''kókkos'', "granule") is in the monotypic family Deinococcaceae, and one genus of three in the order Deinococcales of the bacterial phylum ''Deinococcota'' highly resistant to environmental hazards. These bacteria have thick cell walls that give them Gram-positive stains, but they include a second membrane and so are closer in structure to Gram-negative bacteria. ''Deinococcus'' survive when their DNA is exposed to high doses of gamma and UV radiation. Whereas other bacteria change their structure in the presence of radiation, such as by forming endospores, ''Deinococcus'' tolerate it without changing their cellular form and do not retreat into a hardened structure. They are also characterized by the presence of the carotenoid pigment deinoxanthin that give them their pink color. They are usually isolated according to these two criteria. In August 2020, scientists reported that bacteri ...
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Deinococcus
''Deinococcus'' (from the el, δεινός, ''deinos'', "dreadful, strange" and κόκκος, ''kókkos'', "granule") is in the monotypic family Deinococcaceae, and one genus of three in the order Deinococcales of the bacterial phylum ''Deinococcota'' highly resistant to environmental hazards. These bacteria have thick cell walls that give them Gram-positive stains, but they include a second membrane and so are closer in structure to Gram-negative bacteria. ''Deinococcus'' survive when their DNA is exposed to high doses of gamma and UV radiation. Whereas other bacteria change their structure in the presence of radiation, such as by forming endospores, ''Deinococcus'' tolerate it without changing their cellular form and do not retreat into a hardened structure. They are also characterized by the presence of the carotenoid pigment deinoxanthin that give them their pink color. They are usually isolated according to these two criteria. In August 2020, scientists reported that bacteri ...
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Thermales
''Thermales'' is an order of bacteria belonging to the ''Deinococcota'' phylum. They are particularly resistant to heat, and live in the benthic zone of the Gulf of Mexico. Members of the order ''Thermales'' can be distinguished from all other bacteria through molecular signatures consisting of conserved signature indels (CSIs) and conserved signature proteins (CSPs) that are exclusively present in members of this order. Specifically, six CSIs were identified in the following proteins: DNA topoisomerase I, ABC transporter permease, citrate synthase, phosphoribosylformylglycinamidine synthase, pyruvate dehydrogenase and alpha-glucan phosphorylase. 51 CSPs were also found to be exclusively shared by members of this order. In addition, a 76 aa CSI is present in the protein SecA preprotein translocase which is specific to all members of the order ''Thermales'' as well as ''Hydrogenibacillus schlegelii'' (also an thermophilic species). Based on studies on other CSIs and CSPs, it is ...
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Thermaceae
''Thermales'' is an order of bacteria belonging to the ''Deinococcota'' phylum. They are particularly resistant to heat, and live in the benthic zone of the Gulf of Mexico. Members of the order ''Thermales'' can be distinguished from all other bacteria through molecular signatures consisting of conserved signature indels (CSIs) and conserved signature proteins (CSPs) that are exclusively present in members of this order. Specifically, six CSIs were identified in the following proteins: DNA topoisomerase I, ABC transporter permease, citrate synthase, phosphoribosylformylglycinamidine synthase, pyruvate dehydrogenase and alpha-glucan phosphorylase. 51 CSPs were also found to be exclusively shared by members of this order. In addition, a 76 aa CSI is present in the protein SecA preprotein translocase which is specific to all members of the order ''Thermales'' as well as ''Hydrogenibacillus schlegelii'' (also an thermophilic species). Based on studies on other CSIs and CSPs, it is ...
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Deinobacterium
''Deinobacterium'' is a genus in the ''Deinococcota'' phylum (Bacteria). Not to be confused with ''Deinobacter'', a disused name for '' Deinococcus''. Etymology The name ''Deinobacterium'' derives from: Greek adjective ''deinos (δεινός)'', dreadful, strange; New Latin neuter gender noun ''bacterium'', nominally meaning "a rod", but in effect meaning a bacterium; New Latin masculine gender noun ''Deinobacterium'', strange bacterium. Species The genus contains a single species, namely '' D. chartae'' ( Ekman ''et al''. 2011, (Type species of the genus).; Latin genitive case noun ''chartae'', of/from paper.) This bacterium is: * rod-shaped * non-spore-forming * non-motile, aerobic * oxidase and catalase-positive * radiation-resistant bacterium * pale pink colonies on oligotrophic medium at 12 to 50 °C (optimum 37 to 45 °C) and at pH 6 to 10.3 * peptidoglycan type A3β with L-Orn–Gly–Gly * quinone: MK-8 * CG 67% * isolated from biofilm collected from a Finni ...
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Thermus Aquaticus
''Thermus aquaticus'' is a species of bacteria that can tolerate high temperatures, one of several thermophilic bacteria that belong to the ''Deinococcota'' phylum. It is the source of the heat-resistant enzyme ''Taq'' DNA polymerase, one of the most important enzymes in molecular biology because of its use in the polymerase chain reaction (PCR) DNA amplification technique. History When studies of biological organisms in hot springs began in the 1960s, scientists thought that the life of thermophilic bacteria could not be sustained in temperatures above about . Soon, however, it was discovered that many bacteria in different springs not only survived, but also thrived in higher temperatures. In 1969, Thomas D. Brock and Hudson Freeze of Indiana University reported a new species of thermophilic bacteria which they named ''Thermus aquaticus''. The bacterium was first isolated from Mushroom Spring in the Lower Geyser Basin of Yellowstone National Park, which is near the major Gr ...
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Conserved Signature Indels
Conserved signature inserts and deletions (CSIs) in protein sequences provide an important category of molecular markers for understanding phylogenetic relationships. CSIs, brought about by rare genetic changes, provide useful phylogenetic markers that are generally of defined size and they are flanked on both sides by conserved regions to ensure their reliability. While indels can be arbitrary inserts or deletions, CSIs are defined as only those protein indels that are present within conserved regions of the protein. The CSIs that are restricted to a particular clade or group of species, generally provide good phylogenetic markers of common evolutionary descent. Due to the rarity and highly specific nature of such changes, it is less likely that they could arise independently by either convergent or parallel evolution (i.e. homoplasy) and therefore are likely to represent synapomorphy. Other confounding factors such as differences in evolutionary rates at different sites or among ...
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Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) to a large enough amount to study in detail. PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation; Mullis and biochemist Michael Smith (chemist), Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993. PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of Ancient DNA, ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications ...
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Thermus
''Thermus'' is a genus of thermophilic bacteria. It is one of several bacteria belonging to the ''Deinococcota'' phylum. ''Thermus'' species can be distinguished from other genera in the family ''Thermaceae'' as well as all other bacteria by the presence of eight conserved signature indels (CSIs) found in proteins such as adenylate kinase and replicative DNA helicase as well as 14 conserved signature proteins (CSPs) that are exclusively shared by members of this genus. Phylogeny The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and the National Center for Biotechnology Information (NCBI). Unassigned species: * "'' T. anatoliensis''" Kacagan et al. 2016 * "'' T. caldophilus''" Taguchi et al. 1983 * "'' T. eggertssonii''" Peters 2008 * "'' T. murrieta''" Benner et al. 2006 * "'' T. nonproteolyticus''" 1992 * "'' T. rehai''" Lin et al. 2002 * "'' T. yunnanensis''" Gong et al. 2005 See also * Bacteria * Biotechnol ...
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