Botourmiaviridae
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Botourmiaviridae
''Botourmiaviridae'' is a family of positive-strand RNA viruses which infect plants and fungi. The family includes four genera: '' Ourmiavirus'', ''Botoulivirus'', ''Magoulivirus'' and ''Scleroulivirus''. Members of genus ''Ourmiavirus'' infect plants and the other genera infect fungi. The member viruses have genomes which range from 2900 to 4800 nucleotides. Structure Ourmiaviruses are the only members of the family that have a viral structure. The other members are naked and have no viral envelope or capsid. Ourmiaviruses are plant viruses that have a bacilliform virion composed of a single capsid protein. The virions have a series of discrete lengths from 30 to 62 nm. Genome Members of the family ''Botourmiaviridae'' have positive-sense, single-stranded RNA genomes. Contrary to the hosts they infect (plants and fungus), their genome is concise and has few redundancies. The genome of the genus ''Ourmiavirus'' has three segments that encode the capside protein (CP), ...
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Ourmiavirus
''Ourmiavirus'' is a genus of positive-strand RNA viruses. Cucurbits, cherry, and cassava ''Manihot esculenta'', common name, commonly called cassava (), manioc, or yuca (among numerous regional names), is a woody shrub of the spurge family, Euphorbiaceae, native to South America. Although a perennial plant, cassava is extensively ... serve as natural hosts. There are three species in this genus. Diseases associated with this genus include: OuMV: yellowing and chlorotic spot symptoms. Structure Viruses in the genus ''Ourmiavirus'' are non-enveloped, with icosahedral and bacilliform geometries, and T=1 symmetry. The diameter is around 18 nm, with a length of 30 nm. Genome Genomes are linear and segmented, around 2.8kb in length. Life cycle Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. Replication follows the positive stranded RNA virus replication model. Positive stranded RNA virus transcription is th ...
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Positive-strand RNA Virus
Positive-strand RNA viruses (+ssRNA viruses) are a group of related viruses that have positive-sense, single-stranded genomes made of ribonucleic acid. The positive-sense genome can act as messenger RNA (mRNA) and can be directly translated into viral proteins by the host cell's ribosomes. Positive-strand RNA viruses encode an RNA-dependent RNA polymerase (RdRp) which is used during replication of the genome to synthesize a negative-sense antigenome that is then used as a template to create a new positive-sense viral genome. Positive-strand RNA viruses are divided between the phyla ''Kitrinoviricota'', ''Lenarviricota'', and ''Pisuviricota'' (specifically classes ''Pisoniviricetes'' and '' Stelpavirictes'') all of which are in the kingdom '' Orthornavirae'' and realm '' Riboviria''. They are monophyletic and descended from a common RNA virus ancestor. In the Baltimore classification system, +ssRNA viruses belong to Group IV. Positive-sense RNA viruses include pathogen ...
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RNA-dependent RNA Polymerase
RNA-dependent RNA polymerase (RdRp) or RNA replicase is an enzyme that catalyzes the replication of RNA from an RNA template. Specifically, it catalyzes synthesis of the RNA strand complementary to a given RNA template. This is in contrast to typical DNA-dependent RNA polymerases, which all organisms use to catalyze the transcription of RNA from a DNA template. RdRp is an essential protein encoded in the genomes of most RNA-containing viruses with no DNA stage including SARS-CoV-2. Some eukaryotes also contain RdRps, which are involved in RNA interference and differ structurally from viral RdRps. History Viral RdRps were discovered in the early 1960s from studies on mengovirus and polio virus when it was observed that these viruses were not sensitive to actinomycin D, a drug that inhibits cellular DNA-directed RNA synthesis. This lack of sensitivity suggested that there is a virus-specific enzyme that could copy RNA from an RNA template and not from a DNA template. Distr ...
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Movement Protein
In order for a virus to infect a plant, it must be able to move between cells so it can spread throughout the plant. Plant cell walls make this moving/spreading quite difficult and therefore, for this to occur, movement proteins must be present. A movement protein (MP) is a specific virus-encoded protein that is considered to be a general feature of plant genomes. They allow for local and systemic viral spread throughout a plant. MPs were first studied in the Tobacco Mosaic Virus (TMV) where it was found that viruses were unable to spread without the presence of a specific protein. In general, the plant viruses first, move within the cell from replication sites to the plasmodesmata (PD). Then, the virus is able to go through the PD and spread to other cells. This process is controlled through MPs. Different MPs use different mechanisms and pathways to regulate this spread of some viruses. Nearly all plants express at least one MP, while some can encode many different MPs which help ...
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Capsid
A capsid is the protein shell of a virus, enclosing its genetic material. It consists of several oligomeric (repeating) structural subunits made of protein called protomers. The observable 3-dimensional morphological subunits, which may or may not correspond to individual proteins, are called capsomeres. The proteins making up the capsid are called capsid proteins or viral coat proteins (VCP). The capsid and inner genome is called the nucleocapsid. Capsids are broadly classified according to their structure. The majority of the viruses have capsids with either helical or icosahedral structure. Some viruses, such as bacteriophages, have developed more complicated structures due to constraints of elasticity and electrostatics. The icosahedral shape, which has 20 equilateral triangular faces, approximates a sphere, while the helical shape resembles the shape of a spring, taking the space of a cylinder but not being a cylinder itself. The capsid faces may consist of one or more ...
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Sense (molecular Biology)
In molecular biology and genetics, the sense of a nucleic acid molecule, particularly of a strand of DNA or RNA, refers to the nature of the roles of the strand and its complement in specifying a sequence of amino acids. Depending on the context, sense may have slightly different meanings. For example, negative-sense strand of DNA is equivalent to the template strand, whereas the positive-sense strand is the non-template strand whose nucleotide sequence is equivalent to the sequence of the mRNA transcript. DNA sense Because of the complementary nature of base-pairing between nucleic acid polymers, a double-stranded DNA molecule will be composed of two strands with sequences that are reverse complements of each other. To help molecular biologists specifically identify each strand individually, the two strands are usually differentiated as the "sense" strand and the "antisense" strand. An individual strand of DNA is referred to as positive-sense (also positive (+) or simply sense) ...
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Fungus
A fungus ( : fungi or funguses) is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and molds, as well as the more familiar mushrooms. These organisms are classified as a kingdom, separately from the other eukaryotic kingdoms, which by one traditional classification include Plantae, Animalia, Protozoa, and Chromista. A characteristic that places fungi in a different kingdom from plants, bacteria, and some protists is chitin in their cell walls. Fungi, like animals, are heterotrophs; they acquire their food by absorbing dissolved molecules, typically by secreting digestive enzymes into their environment. Fungi do not photosynthesize. Growth is their means of mobility, except for spores (a few of which are flagellated), which may travel through the air or water. Fungi are the principal decomposers in ecological systems. These and other differences place fungi in a single group of related organisms, named the ''Eumycota'' (''true f ...
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