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Bacillus Megaterium
''Bacillus megaterium'' is a rod-like, Gram-positive, mainly aerobic spore forming bacterium found in widely diverse habitats.De Vos, P. ''et al.'' Bergey's Manual of Systematic Bacteriology: Volume 3: The Firmicutes. ''Springer'' (2009) It has a cell length of up to 4 µm and a diameter of 1.5 µm, which is quite large for a bacteria. The cells often occur in pairs and chains, where the cells are joined together by polysaccharides on the cell walls. In the 1960s, prior to the utilization of ''Bacillus subtilis'' for this purpose, ''B. megaterium'' was the main model organism among Gram-positive bacteria for intensive studies on biochemistry, sporulation and bacteriophages. Recently, its popularity has started increasing in the field of biotechnology for its recombinant protein production capacity.Bunk, B. ''et al.'' A short story about a big magic bug. ''Bioengineered Bugs'' 1:85–91 (2010) This species has been recently transferred into the genus '' Priestia''. The ...
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Sudan Black B
Solvent Black 3 is an azo dye. It is a non-fluorescent, relatively thermostable lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a dark brown to black powder with maximum absorption at 596–605 nm and melting point 120–124 °C. It stains blue-black. Applications Solvent Black 3 is used for a wide variety of commercial applications. In the laboratory, Solvent Black 3 is used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan IV Sudan IV (C24H20N4O) is a lysochrome (fat-soluble dye) diazo dye used for the staining of lipids, triglycerides and lipoproteins on frozen paraffin sections. It has the appearance of reddish brown crystals with melting point 199 °C and maxim .... It can be used to stain some other materials than the other Sudan dyes, as it is not so specific to lipids. It is used is in fingerprint enha ...
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Sodium Chloride
Sodium chloride , commonly known as salt (although sea salt also contains other chemical salts), is an ionic compound with the chemical formula NaCl, representing a 1:1 ratio of sodium and chloride ions. With molar masses of 22.99 and 35.45 g/mol respectively, 100 g of NaCl contains 39.34 g Na and 60.66 g Cl. Sodium chloride is the salt most responsible for the salinity of seawater and of the extracellular fluid of many multicellular organisms. In its edible form, salt (also known as '' table salt'') is commonly used as a condiment and food preservative. Large quantities of sodium chloride are used in many industrial processes, and it is a major source of sodium and chlorine compounds used as feedstocks for further chemical syntheses. Another major application of sodium chloride is de-icing of roadways in sub-freezing weather. Uses In addition to the familiar domestic uses of salt, more dominant applications of the approximately 250 million tonnes per year product ...
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Glucose
Glucose is a simple sugar with the molecular formula . Glucose is overall the most abundant monosaccharide, a subcategory of carbohydrates. Glucose is mainly made by plants and most algae during photosynthesis from water and carbon dioxide, using energy from sunlight, where it is used to make cellulose in cell walls, the most abundant carbohydrate in the world. In energy metabolism, glucose is the most important source of energy in all organisms. Glucose for metabolism is stored as a polymer, in plants mainly as starch and amylopectin, and in animals as glycogen. Glucose circulates in the blood of animals as blood sugar. The naturally occurring form of glucose is -glucose, while -glucose is produced synthetically in comparatively small amounts and is less biologically active. Glucose is a monosaccharide containing six carbon atoms and an aldehyde group, and is therefore an aldohexose. The glucose molecule can exist in an open-chain (acyclic) as well as ring (cyclic) fo ...
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Galleria Mellonella
''Galleria mellonella'', the greater wax moth or honeycomb moth, is a moth of the family Pyralidae. ''G. mellonella'' is found throughout the world. It is one of two species of wax moths, with the other being the lesser wax moth. ''G. mellonella'' eggs are laid in the spring, and they have four life stages. Males are able to generate ultrasonic sound pulses, which, along with pheromones, are used in mating. The larvae of ''G. mellonella'' are also often used as a model organism in research. The greater wax moth is well known for its parasitization of honeybees and their hives. Because of the economic loss caused by this species, several control methods including heat treatment and chemical fumigants such as carbon dioxide have been used. The caterpillar of ''G. mellonella'' has attracted interest for its ability to degrade polyethylene plastic. Geographic range ''G. mellonella'' was first reported as a pest in Asia, but then spread to northern Africa, Great Britain, some pa ...
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Saturnia Pavonia
''Saturnia pavonia'', the small emperor moth, is a moth of the family Saturniidae. It was first described by Carl Linnaeus in his 1758 10th edition of ''Systema Naturae''. Sometimes, the incorrect genus name ''Pavonia'' is still used for this species. This moth occurs throughout the Palearctic region and is the only member of its family to be found in the British Isles, where it is usually called simply the emperor moth. Description The male has a wingspan of about with brown and white forewings marked with red and orange fascia and a bold black and orange eyespot. The hindwings are orange with a similar eyespot. The female is larger with a wingspan of about , but less brightly coloured than the male, being generally grey and white but has all wings marked with eyespots similar to the male. The male flies rapidly during the day from mid-April to late June looking for the rather sluggish females, which usually only fly at night. The species inhabits a range of habitats but ...
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Bee Pollen
Bee pollen, also known as bee bread and ambrosia, is a ball or pellet of field-gathered flower pollen packed by worker honeybees, and used as the primary food source for the hive. It consists of simple sugars, protein, minerals and vitamins, fatty acids, and a small percentage of other components. Bee pollen is stored in brood cells, mixed with saliva, and sealed with a drop of honey. Bee pollen is harvested as food for humans and marketed as having various, but yet unproven, health benefits. Details In honeybees (''Apis'' species) pollen is stored in the chambers of the hives. It differs from field gathered pollen as honey bee secretions induce a fermentation process, where biochemical transformations break down the walls of flower pollen grains and render the nutrients more readily available. Forager bees that gather pollen do not eat it themselves, since they stop producing the proteolytic enzymes necessary to digest it when they transition to foraging. The foragers un ...
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Honey
Honey is a sweet and viscous substance made by several bees, the best-known of which are honey bees. Honey is made and stored to nourish bee colonies. Bees produce honey by gathering and then refining the sugary secretions of plants (primarily floral nectar) or the secretions of other insects, like the honeydew of aphids. This refinement takes place both within individual bees, through regurgitation and enzymatic activity, as well as during storage in the hive, through water evaporation that concentrates the honey's sugars until it is thick and viscous. Honey bees stockpile honey in the hive. Within the hive is a structure made from wax called honeycomb. The honeycomb is made up of hundreds or thousands of hexagonal cells, into which the bees regurgitate honey for storage. Other honey-producing species of bee store the substance in different structures, such as the pots made of wax and resin used by the stingless bee. Honey for human consumption is collected from w ...
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Endophyte
An endophyte is an endosymbiont, often a bacterium or fungus, that lives within a plant for at least part of its life cycle without causing apparent disease. Endophytes are ubiquitous and have been found in all species of plants studied to date; however, most of the endophyte/plant relationships are not well understood. Some endophytes may enhance host growth, nutrient acquisition and improve the plant's ability to tolerate abiotic stresses, such as drought and decrease biotic stresses by enhancing plant resistance to insects, pathogens and herbivores. Although endophytic bacteria and fungi are frequently studied, endophytic archaea are increasingly being considered for their role in plant growth promotion as part of the core microbiome of a plant. History Endophytes were first described by the German botanist Johann Heinrich Friedrich Link in 1809. They were thought to be plant parasitic fungi and they were later termed as "microzymas" by the French scientist Béchamp. There wa ...
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Bacillus Cereus
''Bacillus cereus'' is a Gram-positive rod-shaped bacterium commonly found in soil, food, and marine sponges. The specific name, ''cereus'', meaning "waxy" in Latin, refers to the appearance of colonies grown on blood agar. Some strains are harmful to humans and cause foodborne illness due to their spore-forming nature, while other strains can be beneficial as probiotics for animals, and even exhibit mutualism with certain plants. ''B. cereus'' bacteria may be anaerobes or facultative anaerobes, and like other members of the genus ''Bacillus'', can produce protective endospores. They have a wide range of virulence factors, including phospholipase C, cereulide, sphingomyelinase, metalloproteases, and cytotoxin K, many of which are regulated via quorum sensing. ''B. cereus'' strains exhibit flagellar motility. The ''Bacillus cereus'' group comprises seven closely related species: ''B. cereus'' ''sensu stricto'' (referred to herein as ''B. cereus''), '' B.  ...
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Bacillus Anthracis
''Bacillus anthracis'' is a gram-positive and rod-shaped bacterium that causes anthrax, a deadly disease to livestock and, occasionally, to humans. It is the only permanent ( obligate) pathogen within the genus '' Bacillus''. Its infection is a type of zoonosis, as it is transmitted from animals to humans. It was discovered by a German physician Robert Koch in 1876, and became the first bacterium to be experimentally shown as a pathogen. The discovery was also the first scientific evidence for the germ theory of diseases. ''B. anthracis'' measures about 3 to 5 μm long and 1 to 1.2 μm wide. The reference genome consists of a 5,227,419 bp circular chromosome and two extrachromosomal DNA plasmids, pXO1 and pXO2, of 181,677 and 94,830 bp respectively, which are responsible for the pathogenicity. It forms a protective layer called endospore by which it can remain inactive for many years and suddenly becomes infective under suitable environmental conditions. Because of the resil ...
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Bacillus Flexus
''Bacillus flexus'' is an aerobic, Gram-variable, rod-shaped, endospore-forming, oxidase positive bacteria. The endospores are ellipsoidal, located in central/paracentral, unswollen sporangia. In laboratory conditions, it produces opaque, creamish, raised margin colonies at 30  when incubated at 24–72 hrs. on Tryptic Soy Agar (TSA). These bacteria may be isolated from feces (poultry) and soil. Human pathogenicity has not been well described at this time. This species has been recently transferred into the genus ''Priestia ''Priestia'' is a genus of mostly Gram-Positive ( ''Priestia flexa'' stains Gram-variable and ''Priestia koreensis'' stains Gram-negative) rod-shaped bacteria in the family ''Bacillaceae'' from the order ''Bacillales.'' The type species of this ge ....'' The correct nomenclature is ''Priestia flexa.'' References flexus {{firmicutes-stub ...
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16S Ribosomal RNA
16 S ribosomal RNA (or 16 S rRNA) is the RNA component of the 30S subunit of a prokaryotic ribosome ( SSU rRNA). It binds to the Shine-Dalgarno sequence and provides most of the SSU structure. The genes coding for it are referred to as 16S rRNA gene and are used in reconstructing phylogenies, due to the slow rates of evolution of this region of the gene. Carl Woese and George E. Fox were two of the people who pioneered the use of 16S rRNA in phylogenetics in 1977. Multiple sequences of the 16S rRNA gene can exist within a single bacterium. Functions * Like the large (23S) ribosomal RNA, it has a structural role, acting as a scaffold defining the positions of the ribosomal proteins. * The 3-end contains the anti- Shine-Dalgarno sequence, which binds upstream to the AUG start codon on the mRNA. The 3-end of 16S RNA binds to the proteins S1 and S21 which are known to be involved in initiation of protein synthesis * Interacts with 23S, aiding in the binding of the two riboso ...
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