Blotto (other)
Blotto may refer to: * Blotto (biology), a reagent used in immunological assays * Blotto, a colloquial term meaning drunkenness * ''Blotto'' (film) a 1930 Laurel and Hardy short comedy film * Blotto (band), an Albany, NY, rock band in the late 1970s and early 1980s * Blotto games A Colonel Blotto game is a type of two-person constant-sum game in which the players (officers) are tasked to simultaneously distribute limited resources over several objects (battlefields). In the classic version of the game, the player devoting ...

Blotto (biology)
In biology, BLOTTO is a blocking reagent made from nonfat dry milk, phosphate buffered saline, and sodium azide. Its name is an almost-acronym of bovine lacto transfer technique optimizer. It constitutes an inexpensive source of nonspecific protein (milk casein) which blocks protein binding sites in a variety of experimental paradigms, notably Southern blots, Western blots, and ELISA. Its use was first reported in 1984 by Johnson and Elder's lab at Scripps. Prior to 1984, partially purified proteins such as bovine serum albumin, ovalbumin, or gelatin Gelatin or gelatine (from la, gelatus meaning "stiff" or "frozen") is a translucent, colorless, flavorless food ingredient, commonly derived from collagen taken from animal body parts. It is brittle when dry and rubbery when moist. It may also ... from various species had been used as blocking reagents but had the disadvantage of being expensive. References Immunology {{molecular-biology-stub ...
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Drunkenness
Alcohol intoxication, also known as alcohol poisoning, commonly described as drunkenness or inebriation, is the negative behavior and physical effects caused by a recent consumption of alcohol. In addition to the toxicity of ethanol, the main psychoactive component of alcoholic beverages, other physiological symptoms may arise from the activity of acetaldehyde, a metabolite of alcohol. These effects may not arise until hours after ingestion and may contribute to the condition colloquially known as a hangover. Symptoms of intoxication at lower doses may include mild sedation and poor coordination. At higher doses, there may be slurred speech, trouble walking, and vomiting. Extreme doses may result in a respiratory depression, coma, or death. Complications may include seizures, aspiration pneumonia, injuries including suicide, and low blood sugar. Alcohol intoxication can lead to alcohol-related crime with perpetrators more likely to be intoxicated than victims. Alcohol into ...
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Blotto (film)
''Blotto'' is a 1930 American pre-Code comedy film directed by James Parrott and starring Stan Laurel and Oliver Hardy. The short was produced by Hal Roach and originally distributed by Metro-Goldwyn-Mayer. The name of the film relates to the now archaic term "to be blotto" meaning to be very drunk. Plot Set during the Prohibition era, Laurel and Hardy make plans to spend a wild night out at the Rainbow Club. Stan is at home, itching to go out, while his wife nags his every movement. Phoning Stan at home, Ollie at first continually gets Stan saying "wrong number" because his wife is watching. He then suggests a ruse by which Stan is to convince his wife, who keeps him on a short leash, that he has been called away on business. Stan readily agrees to the idea, assuring Ollie that his wife is "so dumb she'll never know the difference". Mrs. Laurel, eavesdropping on a second line in the bedroom, is furious, but continues listening as Stan tells Ollie that he knows where he can ...
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Blotto (band)
Blotto was an American rock band from Albany, New York known for mixing music and humor. They formed in 1978 from the Star Spangled Washboard Band, a comedy jug band whom ''The New York Times'' described as "reminiscent of collision between the Earl Scruggs Review and the Three Stooges." Blotto's music combined new wave and soul/ R&B however it had comedic themes. It began as a pick-up band in Saratoga Springs at a club then known as 17 Maple Ave. (later The Metro), with three Washboard Band alumni (who took the stage names Bowtie Blotto, Sergeant Blotto, and Broadway Blotto), plus a drummer and a bassist (Lee Harvey Blotto, and Cheese Blotto). They were joined by Blanche Blotto, who contributed vocals and keyboards and inspired the band's drumhead lady logo. They began to amass a following and played in the New York City area at clubs such as The Ritz, SNAFU, Eighty-Eight, and My Father's Place. DJ Vin Scelsa of WNEW-FM picked up on their initial recording of "I Wanna Be A Life ...
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