Annwoodia Aquaesulis
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Annwoodia Aquaesulis
The genus ''Annwoodia'' was named in 2017 to circumscribe an organism previously described as a member of the genus ''Thiobacillus'', ''Thiobacillus aquaesulis'' - the type and only species is ''Annwoodia aquaesulis'', which was isolated from the geothermal waters of the Roman Baths in the city of Bath in the United Kingdom by Ann P. Wood and Donovan P. Kelly of the University of Warwick - the genus was subsequently named to honour Wood's contribution to microbiology. The genus falls within the family '' Thiobacillaceae'' along with ''Thiobacillus'' and '' Sulfuritortus'', both of which comprise autotrophic organisms dependent on thiosulfate, other sulfur oxyanions and sulfide as electron donors for chemolithoheterotrophic growth. Whilst ''Annwoodia'' spp. and '' Sulfuritortus'' spp. are thermophilic, ''Thiobacillus'' spp. are mesophilic. ''A. aquaesulis'' is moderately thermophilic with an optimum temperature of 43 °C and a temperature range of 30 °C to 55 °C ...
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Bacteria
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationsh ...
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Sulfur
Sulfur (or sulphur in British English) is a chemical element with the symbol S and atomic number 16. It is abundant, multivalent and nonmetallic. Under normal conditions, sulfur atoms form cyclic octatomic molecules with a chemical formula S8. Elemental sulfur is a bright yellow, crystalline solid at room temperature. Sulfur is the tenth most abundant element by mass in the universe and the fifth most on Earth. Though sometimes found in pure, native form, sulfur on Earth usually occurs as sulfide and sulfate minerals. Being abundant in native form, sulfur was known in ancient times, being mentioned for its uses in ancient India, ancient Greece, China, and ancient Egypt. Historically and in literature sulfur is also called brimstone, which means "burning stone". Today, almost all elemental sulfur is produced as a byproduct of removing sulfur-containing contaminants from natural gas and petroleum.. Downloahere The greatest commercial use of the element is the production o ...
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Oxygen
Oxygen is the chemical element with the symbol O and atomic number 8. It is a member of the chalcogen group in the periodic table, a highly reactive nonmetal, and an oxidizing agent that readily forms oxides with most elements as well as with other compounds. Oxygen is Earth's most abundant element, and after hydrogen and helium, it is the third-most abundant element in the universe. At standard temperature and pressure, two atoms of the element bind to form dioxygen, a colorless and odorless diatomic gas with the formula . Diatomic oxygen gas currently constitutes 20.95% of the Earth's atmosphere, though this has changed considerably over long periods of time. Oxygen makes up almost half of the Earth's crust in the form of oxides.Atkins, P.; Jones, L.; Laverman, L. (2016).''Chemical Principles'', 7th edition. Freeman. Many major classes of organic molecules in living organisms contain oxygen atoms, such as proteins, nucleic acids, carbohydrates, and fats, as ...
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Terminal Electron Acceptor
An electron acceptor is a chemical entity that accepts electrons transferred to it from another compound. It is an oxidizing agent that, by virtue of its accepting electrons, is itself reduced in the process. Electron acceptors are sometimes mistakenly called electron receptors. Typical oxidizing agents undergo permanent chemical alteration through covalent or ionic reaction chemistry, resulting in the complete and irreversible transfer of one or more electrons. In many chemical circumstances, however, the transfer of electronic charge from an electron donor may be only fractional, meaning an electron is not completely transferred, but results in an electron resonance between the donor and acceptor. This leads to the formation of charge transfer complexes in which the components largely retain their chemical identities. The electron accepting power of an acceptor molecule is measured by its electron affinity which is the energy released when filling the lowest unoccupied molecul ...
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Nitrate
Nitrate is a polyatomic ion A polyatomic ion, also known as a molecular ion, is a covalent bonded set of two or more atoms, or of a metal complex, that can be considered to behave as a single unit and that has a net charge that is not zero. The term molecule may or may no ... with the chemical formula . salt (chemistry), Salts containing this ion are called nitrates. Nitrates are common components of fertilizers and explosives. Almost all inorganic nitrates are solubility, soluble in water. An example of an insoluble nitrate is bismuth oxynitrate. Structure The ion is the conjugate acid, conjugate base of nitric acid, consisting of one central nitrogen atom surrounded by three identically bonded oxygen atoms in a trigonal planar arrangement. The nitrate ion carries a formal charge of −1. This charge results from a combination formal charge in which each of the three oxygens carries a − charge, whereas the nitrogen carries a +1 charge, all these adding up to formal c ...
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Chemostat
A chemostat (from ''chem''ical environment is ''stat''ic) is a bioreactor to which fresh medium is continuously added, while culture liquid containing left over nutrients, metabolic end products and microorganisms is continuously removed at the same rate to keep the culture volume constant. By changing the rate with which medium is added to the bioreactor the specific growth rate of the microorganism can be easily controlled within limits. Operation Steady state One of the most important features of chemostats is that microorganisms can be grown in a physiological steady state under constant environmental conditions. In this steady state, growth occurs at a constant specific growth rate and all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.). In addition, environmental conditions can be controlled by the experimenter. Microorganisms growing in chemostats usually reach a steady stat ...
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Nutrient Broth
Nutrient agar is a general purpose liquid medium supporting growth of a wide range of non-fastidious organisms. It typically contains ( mass/volume): * 0.5% peptone - this provides organic nitrogen * 0.3% beef extract/yeast extract - the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts * 1.5% agar - this gives the mixture solidity * 0.5% sodium chloride - this gives the mixture proportions similar to those found in the cytoplasm of most organisms * distilled water - water serves as a transport medium for the agar's various substances * pH adjusted to neutral (6.8) at . Nutrient broth has the same composition,but lacks agar. These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at for 15 minutes. Then they are cooled to around and poured into Petri dishes which are covered immediately. Once the dishes hold solidified agar, they are stored upside d ...
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Tetrathionate
The tetrathionate anion, , is a sulfur oxoanion derived from the compound tetrathionic acid, H2S4O6. Two of the sulfur atoms present in the ion are in oxidation state 0 and two are in oxidation state +5. Alternatively, the compound can be viewed as the adduct resulting from the binding of to SO3. Tetrathionate is one of the polythionates, a family of anions with the formula ''n''(SO3)2sup>2−. Its IUPAC name is ''2-(dithioperoxy)disulfate'', and the name of its corresponding acid is ''2-(dithioperoxy)disulfuric acid''. The Chemical Abstracts Service identifies tetrathionate by the CAS Number 15536-54-6. Formation Tetrathionate is a product of the oxidation of thiosulfate, , by iodine, I2: :2 + I2 → + 2 I− Structure Tetrathionate's structure can be visualized by following three edges of a rectangular cuboid, as in the diagram below. The structure shown is the configuration of in BaS4O6·2H2O and Na2S4O6·2H2O. Dihedral S–S–S–S angles approaching 90° are comm ...
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Bicarbonate
In inorganic chemistry, bicarbonate (IUPAC-recommended nomenclature: hydrogencarbonate) is an intermediate form in the deprotonation of carbonic acid. It is a polyatomic anion with the chemical formula . Bicarbonate serves a crucial biochemical role in the physiological pH buffering system. The term "bicarbonate" was coined in 1814 by the English chemist William Hyde Wollaston. The name lives on as a trivial name. Chemical properties The bicarbonate ion (hydrogencarbonate ion) is an anion with the empirical formula and a molecular mass of 61.01  daltons; it consists of one central carbon atom surrounded by three oxygen atoms in a trigonal planar arrangement, with a hydrogen atom attached to one of the oxygens. It is isoelectronic with nitric acid . The bicarbonate ion carries a negative one formal charge and is an amphiprotic species which has both acidic and basic properties. It is both the conjugate base of carbonic acid ; and the conjugate acid of , ...
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Carbon Dioxide
Carbon dioxide (chemical formula ) is a chemical compound made up of molecules that each have one carbon atom covalently double bonded to two oxygen atoms. It is found in the gas state at room temperature. In the air, carbon dioxide is transparent to visible light but absorbs infrared radiation, acting as a greenhouse gas. It is a trace gas in Earth's atmosphere at 421 parts per million (ppm), or about 0.04% by volume (as of May 2022), having risen from pre-industrial levels of 280 ppm. Burning fossil fuels is the primary cause of these increased CO2 concentrations and also the primary cause of climate change.IPCC (2022Summary for policy makersiClimate Change 2022: Mitigation of Climate Change. Contribution of Working Group III to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA Carbon dioxide is soluble in water and is found in groundwater, lakes, ice caps, ...
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Electron Donor
In chemistry, an electron donor is a chemical entity that donates electrons to another compound. It is a reducing agent that, by virtue of its donating electrons, is itself oxidized in the process. Typical reducing agents undergo permanent chemical alteration through covalent or ionic reaction chemistry. This results in the complete and irreversible transfer of one or more electrons. In many chemical circumstances, however, the transfer of electronic charge to an electron acceptor may be only fractional, meaning an electron is not completely transferred, but results in an electron resonance between the donor and acceptor. This leads to the formation of charge transfer complexes in which the components largely retain their chemical identities. The electron donating power of a donor molecule is measured by its ionization potential which is the energy required to remove an electron from the highest occupied molecular orbital (HOMO). The overall energy balance (ΔE), i.e., energ ...
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Chemolithoautotroph
A lithoautotroph is an organism which derives energy from reactions of reduced compounds of mineral (inorganic) origin. Two types of lithoautotrophs are distinguished by their energy source; photolithoautotrophs derive their energy from light while chemolithoautotrophs (chemolithotrophs or chemoautotrophs) derive their energy from chemical reactions. Chemolithoautotrophs are exclusively microbes. Photolithoautotrophs include macroflora such as plants; these do not possess the ability to use mineral sources of reduced compounds for energy. Most chemolithoautotrophs belong to the domain Bacteria, while some belong to the domain Archaea. Lithoautotrophic bacteria can only use inorganic molecules as substrates in their energy-releasing reactions. The term "lithotroph" is from Greek ''lithos'' (''λίθος'') meaning "rock" and ''trōphos'' (τροφοσ) meaning "consumer"; literally, it may be read "eaters of rock". The "lithotroph" part of the name refers to the fact that these org ...
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