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Ashdown's Medium
Ashdown's medium is a selective culture medium for the isolation and characterisation of ''Burkholderia pseudomallei'' (the bacterium that causes melioidosis). Ashdown's medium was first described by LR Ashdown in 1979. It is used for the selective isolation of ''B. pseudomallei'' from clinical specimens taken from non-sterile sites (e.g., sputum) as well as to produce the characteristic morphology of ''B. pseudomallei''. The medium contains crystal violet and gentamicin as selective agents to suppress the growth of other bacteria. Colonies of ''B. pseudomallei'' also take up neutral red Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red. It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many stai ... which is present in the medium, and this further helps to distinguish it from other bacteria. Gentamicin slightly inhibits the growth of ''B. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bps Close
BPS, Bps or bps may refer to: Science and mathematics *Plural of bp, base pair, a measure of length of DNA *Plural of bp, basis point, one one-hundredth of a percentage point - ‱ *Battered person syndrome, a physical and psychological condition found in victims of abuse *Best practice statement, a qualification of a method used in guidelines documents *Bisphenol S, an organic chemical compound *Bladder pain syndrome, a disorder characterised by pain associated with urination *Bogomol'nyi–Prasad–Sommerfield bound, a mathematical concept in field and string theory *Bogomol'nyi–Prasad–Sommerfield state, solutions saturating the BPS bound * BPS domain, a protein domain *Bronchopulmonary sequestration, where a section of lung tissue has a decreased blood supply * Bovine papular stomatitis, a zoonotic farmyard pox Computing *IBM Basic Programming Support, BPS/360 *Bits per second (bps), a data rate unit *Bytes per second (Bps), a data rate unit *Bits per sample (bps), referrin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Growth Medium
A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss ''Physcomitrella patens''. Different types of media are used for growing different types of cells. The two major types of growth media are those used for cell culture, which use specific cell types derived from plants or animals, and those used for microbiological culture, which are used for growing microorganisms such as bacteria or fungi. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellular parasites and require a growth medium containing living cells. Types The most common growth media ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Burkholderia Pseudomallei
''Burkholderia pseudomallei'' (also known as ''Pseudomonas pseudomallei'') is a Gram-negative, bipolar, aerobic, motile rod-shaped bacterium. It is a soil-dwelling bacterium endemic in tropical and subtropical regions worldwide, particularly in Thailand and northern Australia. It was reported in 2008 that there had been an expansion of the affected regions due to significant natural disasters, and it could be found in Southern China, Hong Kong, and countries in America. ''B. pseudomallei'', amongst other pathogens, has been found in monkeys imported into the United States from Asia for laboratory use, posing a risk that the pathogen could be introduced into the country. Although it is mainly a soil-dwelling bacteria, a study performed by Apinya Pumpuang and others showed that ''Burkholderia pseudomallei'' survived in distilled water for 16 years, demonstrating that it is capable of living in water if a specific environment is provided. It is resistant to a variety of harsh condit ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Melioidosis
Melioidosis is an infectious disease caused by a gram-negative bacterium called ''Burkholderia pseudomallei''. Most people exposed to ''B. pseudomallei'' experience no symptoms; however, those who do experience symptoms have signs and symptoms that range from mild such as fever and skin changes, to severe with pneumonia, abscesses, and septic shock that could cause death. Approximately 10% of people with melioidosis develop symptoms that last longer than two months, termed "chronic melioidosis". Humans are infected with ''B. pseudomallei'' by contact with contaminated soil or water. The bacteria enter the body through wounds, inhalation, or ingestion. Person-to-person or animal-to-human transmission is extremely rare. The infection is constantly present in Southeast Asia particularly in northeast Thailand and northern Australia. In temperate countries such as Europe and the United States, melioidosis cases are usually imported from countries where melioidosis is endemic. The sign ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sputum
Sputum is mucus that is coughed up from the lower airways (the trachea and bronchi). In medicine, sputum samples are usually used for a naked eye examination, microbiological investigation of respiratory infections and cytological investigations of respiratory systems. It is crucial that the specimen does not include any mucoid material from the nose or oral cavity. A naked eye exam of the sputum can be done at home by a patient in order to note the various colors (see below). Any hint of yellow or green color (pus) suggests an airway infection (but does not indicate the type of organism causing it). Such color hints are best detected when the sputum is viewed on a very white background such as white paper, a white pot or a white sink surface. The more intense the yellow color, the more likely it is a caused by an infection (bronchitis, bronchopneumonia or pneumonia). Having green, yellow, or thickened phlegm (sputum) does not always indicate the presence of an infection. Also, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Crystal Violet
Crystal violet or gentian violet, also known as methyl violet 10B or hexamethyl pararosaniline chloride, is a triarylmethane dye used as a histological stain and in Gram's method of classifying bacteria. Crystal violet has antibacterial, antifungal, and anthelmintic ( vermicide) properties and was formerly important as a topical antiseptic. The medical use of the dye has been largely superseded by more modern drugs, although it is still listed by the World Health Organization. The name ''gentian violet'' was originally used for a mixture of methyl pararosaniline dyes (methyl violet), but is now often considered a synonym for ''crystal violet''. The name refers to its colour, being like that of the petals of certain gentian flowers; it is not made from gentians or violets. Production A number of possible routes can be used to prepare crystal violet. The original procedure developed by the German chemists Kern and Caro involved the reaction of dimethylaniline with phosgene to gi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gentamicin
Gentamicin is an antibiotic used to treat several types of bacterial infections. This may include bone infections, endocarditis, pelvic inflammatory disease, meningitis, pneumonia, urinary tract infections, and sepsis among others. It is not effective for gonorrhea or chlamydia infections. It can be given intravenously, by intramuscular injection, or topically. Topical formulations may be used in burns or for infections of the outside of the eye. It is often only used for two days until bacterial cultures determine what specific antibiotics the infection is sensitive to. The dose required should be monitored by blood testing. Gentamicin can cause inner ear problems and kidney problems. The inner ear problems can include problems with balance and hearing loss. These problems may be permanent. If used during pregnancy, it can cause harm to the developing baby. However, it appears to be safe for use during breastfeeding. Gentamicin is a type of aminoglycoside. It works by disru ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Neutral Red
Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red. It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods. Together with Janus Green B, it is used to stain embryonal tissues and supravital staining of blood. Can be used for staining Golgi apparatus in cells and Nissl granules in neurons. In microbiology, it is used in the MacConkey agar to differentiate bacteria for lactose fermentation.Neutral redcan be used as a vital stain. The Neutral Red Cytotoxicity Assay was first developed by Ellen Borenfreund in 1984. In the Neutral Red Assay live cells incorporate neutral red into their lysosomes. As cells begin to die, their ability to incorporate neutral red diminishes. Thus, loss of neutral red uptake corresponds to loss of cell viability. The neutral red is also used to stain cell cultures for plate titration of viruses. Neutral red ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
