Alexa Dyes
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Alexa Dyes
The Alexa Fluor family of fluorescence, fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Life Technologies (Thermo Fisher Scientific), Thermo Fisher Scientific, and sold under the Invitrogen brand name. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscope, fluorescence microscopy and cell biology. Alexa Fluor dyes can be conjugated directly to Primary and secondary antibodies, primary antibodies or to Primary and secondary antibodies, secondary antibodies to amplify signal and sensitivity or other biomolecules. The Excited state, excitation and Emission (electromagnetic radiation), emission spectra of the Alexa Fluor series cover the visible spectrum and extend into the infrared. The individual members of the family are numbered according roughly to their excitation maxima in nanometers. History Dick Haugland, Richard and Rosaria Haugland, the founders of Molecular Probes, are well known in biology and chemist ...
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Fluorescence
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, than the absorbed radiation. A perceptible example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the electromagnetic spectrum (invisible to the human eye), while the emitted light is in the visible region; this gives the fluorescent substance a distinct color that can only be seen when the substance has been exposed to UV light. Fluorescent materials cease to glow nearly immediately when the radiation source stops, unlike phosphorescent materials, which continue to emit light for some time after. Fluorescence has many practical applications, including mineralogy, gemology, medicine, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, vacu ...
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