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A Gunfighter's Pledge
''A Gunfighter's Pledge'' (the working title was ''The Pledge'') is a 2008 American Western television film starring Luke Perry. The film premiered on Hallmark Channel in July 2008. It was filmed at Big Sky Ranch in Simi Valley, California. Plot Ex-lawman Matt Austin (Luke Perry) accidentally kills an innocent man ( Alex Paez) while hunting an outlaw who killed his family. He pledges to the dying man that he will take his body to his sister Amaya (Jaclyn DeSantis). Austin wrestles with the idea of telling her he caused the death, but is faced with bigger battles once at the farm. There he gets involved with land baron Horn (C. Thomas Howell), who is trying to take Amaya's land. Their lives are more intertwined than he thought, when he takes on Horn and discovers that Horn has hired the man he was looking for all along. Cast * Luke Perry as Matt Austin * C. Thomas Howell as Horn * Kim Coates as Tate * Jaclyn DeSantis as Amaya * Francesco Quinn as Sheriff * Jorge-Luis Pallo as ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Guerrilla Marketing
Guerrilla marketing is an advertisement strategy in which a company uses surprise and/or unconventional interactions in order to promote a product or service. It is a type of publicity. The term was popularized by Jay Conrad Levinson's 1984 book ''Guerrilla Marketing''. Guerrilla marketing uses multiple techniques and practices in order to establish direct contact with potential customers. One of the goals of this interaction is to cause an emotional reaction in the clients, and the ultimate goal of marketing is to induce people to remember products or brands in a different way than they might have been accustomed to. As traditional advertising media channelssuch as print, radio, television, and direct maillose popularity, marketers and advertisers have felt compelled to find new strategies to convey their commercial messages to the consumer. Guerrilla marketing focuses on taking the consumer by surprise to make a dramatic impression about the product or brand. This in tur ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chip Sickler
Chromatin immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the cell. It aims to determine whether specific proteins are associated with specific genomic regions, such as transcription factors on promoters or other DNA binding sites, and possibly define cistromes. ChIP also aims to determine the specific location in the genome that various histone modifications are associated with, indicating the target of the histone modifiers. ChIP is crucial for the advancements in the field of epigenomics and learning more about epigenetic phenomena. Briefly, the conventional method is as follows: # DNA and associated proteins on chromatin in living cells or tissues are crosslinked (this step is omitted in Native ChIP). # The DNA-protein complexes (chromatin-protein) are then sheared into ~500 bp DNA fragments by sonication or nuclease digestion. # Cross-linked DNA fragments associated with the prot ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
New York City
New York, often called New York City or NYC, is the List of United States cities by population, most populous city in the United States. With a 2020 population of 8,804,190 distributed over , New York City is also the List of United States cities by population density, most densely populated major city in the United States, and is more than twice as populous as second-place Los Angeles. New York City lies at the southern tip of New York (state), New York State, and constitutes the geographical and demographic center of both the Northeast megalopolis and the New York metropolitan area, the largest metropolitan area in the world by urban area, urban landmass. With over 20.1 million people in its metropolitan statistical area and 23.5 million in its combined statistical area as of 2020, New York is one of the world's most populous Megacity, megacities, and over 58 million people live within of the city. New York City is a global city, global Culture of New ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rockefeller Plaza
Rockefeller Center is a large complex consisting of 19 commerce, commercial buildings covering between 48th Street (Manhattan), 48th Street and 51st Street (Manhattan), 51st Street in Midtown Manhattan, New York City. The 14 original Art Deco buildings, commissioned by the Rockefeller family, span the area between Fifth Avenue and Sixth Avenue (Manhattan), Sixth Avenue, split by a large sunken square and a private street called Rockefeller Plaza. Later additions include 75 Rockefeller Plaza across 51st Street at the north end of Rockefeller Plaza, and four International Style (architecture), International Style buildings on the west side of Sixth Avenue. In 1928, the site's then-owner, Columbia University, leased the land to John D. Rockefeller Jr., who was the main person behind the complex's construction. Originally envisioned as the site for a new Metropolitan Opera building, the current Rockefeller Center came about after the Met could not afford to move to the proposed new ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Times Square
Times Square is a major commercial intersection, tourist destination, entertainment hub, and neighborhood in Midtown Manhattan, New York City. It is formed by the junction of Broadway, Seventh Avenue, and 42nd Street. Together with adjacent Duffy Square, Times Square is a bowtie-shaped space five blocks long between 42nd and 47th Streets. Brightly lit at all hours by numerous digital billboards and advertisements as well as businesses offering 24/7 service, Times Square is sometimes referred to as "the Crossroads of the World", "the Center of the Universe", "the heart of the Great White Way", “the Center of the Entertainment Universe”, and "the heart of the world". One of the world's busiest pedestrian areas, it is also the hub of the Broadway Theater District and a major center of the world's entertainment industry. Times Square is one of the world's most visited tourist attractions, drawing an estimated 50 million visitors annually. Approximately 330,000 people ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cowboy
A cowboy is an animal herder who tends cattle on ranches in North America, traditionally on horseback, and often performs a multitude of other ranch-related tasks. The historic American cowboy of the late 19th century arose from the '' vaquero'' traditions of northern Mexico and became a figure of special significance and legend.Malone, J., p. 1. A subtype, called a wrangler, specifically tends the horses used to work cattle. In addition to ranch work, some cowboys work for or participate in rodeos. Cowgirls, first defined as such in the late 19th century, had a less-well documented historical role, but in the modern world work at identical tasks and have obtained considerable respect for their achievements. Cattle handlers in many other parts of the world, particularly South America and Australia, perform work similar to the cowboy. The cowboy has deep historic roots tracing back to Spain and the earliest European settlers of the Americas. Over the centuries, differences ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Guerilla Marketing
''Guerilla Marketing'' ( si, ගරිල්ලා මාර්කටිං, italic=yes) is a 2005 Sri Lankan Sinhala action thriller film directed by Jayantha Chandrasiri and produced by K.C.K Communications for Euroshian Consultancy. It stars Yashoda Wimaladharma, Kamal Addararachchi and Sangeetha Weeraratne in lead roles along with Jackson Anthony and Sriyantha Mendis. Music composed by Premasiri Khemadasa. It is the 1050th Sri Lankan film in the Sinhala cinema. Plot Thisara is running an advertising agency. He has had a relationship with his cousin Suramya. But when she was away for her education he married Rangi, a young woman from a rich family. When Suramya returned after completing her education, she joins Thisara's agency, even though she is qualified to join a better firm. Suramya seems to be traditionally dressed but well talented, educated and professional while Rangi is western styled but not qualified or educated despite her richness. Presidential election is to be c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Laura Molina (artist)
Laura Molina (born December 15, 1957) is an American artist, musician, and actress from Los Angeles, California. Molina is perhaps best known for her ''Naked Dave'' paintings. She is also the creator of '' Cihualyaomiquiz, The Jaguar'', a self-published comic book printed under Molina's own Insurgent Comix imprint. Career Art career Molina's distinct style is very much influenced by art associated with the Chicano Movement of the 1960s, Mexican culture, especially Frida Kahlo, 20th century Mexican Calendar artist, Jesus Helguera and the British Pre-Raphaelites. Her projects have included the Naked Dave series of paintings and a self-published comic book, "''Cihualyaomiquiz, The Jaguar.''" She was an Artist-in-Residence at Self Help Graphics & Art from 1993 through 1995 and participated in the Screen Print Atelier in 2003 & 2006. "She was a woman with great artistic talent", says Barney Dino. In 2006, Molina founded Chicano Art Magazine and served as its first Editor-in-Chief. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Laci Greenfield
The ''lac'' repressor (LacI) is a DNA-binding protein that inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. These genes are repressed when lactose is not available to the cell, ensuring that the bacterium only invests energy in the production of machinery necessary for uptake and utilization of lactose when lactose is present. When lactose becomes available, it is firstly converted into allolactose by β-Galactosidase (lacZ) in bacteria. The DNA binding ability of lac repressor bound with allolactose is inhibited due to allosteric regulation, thereby genes coding for proteins involved in lactose uptake and utilization can be expressed. Function The ''lac'' repressor (LacI) operates by a helix-turn-helix motif in its DNA-binding domain, binding base-specifically to the major groove of the operator region of the ''lac'' operon, with base contacts also made by residues of symmetry-related alpha helices, the "hinge" heli ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Miranda Cunha
Miranda may refer to: Law * ''Miranda v. Arizona'', an American legal case * ''Miranda'' warning, an American police warning given to suspects about their rights, before they are interrogated Places Australia * Miranda, New South Wales * Miranda railway station, New South Wales Portugal * Miranda do Corvo, a ''município'' in Coimbra District, Centro * Miranda do Douro (parish), a ''freguesia'' in Bragança District, Norte * Miranda do Douro, a ''município'' in Bragança District, Norte * Terra de Miranda, a plateau in Bragança District, Norte Spain * Miranda (Avilés), a parish of Avilés, Asturias * Belmonte de Miranda, Asturias * Miranda de Arga, Navarre * Miranda de Ebro, Castile and Leon * , in Los Rábanos, in the Province of Soria, Castile and Leon * Miranda del Castañar, in the Province of Salamanca, Castile and Leon United States * Miranda, California * Miranda, South Dakota Venezuela * Miranda (state) * Francisco de Miranda Municipality, Anzoátegui * Francisco ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Jordan Timsit
Jordan ( ar, الأردن; tr. ' ), officially the Hashemite Kingdom of Jordan,; tr. ' is a country in Western Asia. It is situated at the crossroads of Asia, Africa, and Europe, within the Levant region, on the East Bank of the Jordan River. Jordan is bordered by Saudi Arabia to the south and east, Iraq to the northeast, Syria to the north, and the Palestinian West Bank, Israel, and the Dead Sea to the west. It has a coastline in its southwest on the Gulf of Aqaba's Red Sea, which separates Jordan from Egypt. Amman is Jordan's capital and largest city, as well as its economic, political, and cultural centre. Modern-day Jordan has been inhabited by humans since the Paleolithic period. Three stable kingdoms emerged there at the end of the Bronze Age: Ammon, Moab and Edom. In the third century BC, the Arab Nabataeans established their Kingdom with Petra as the capital. Later rulers of the Transjordan region include the Assyrian, Babylonian, Roman, Byzantine, Rashidun, Umayya ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bob Ross
Robert Norman Ross (October 29, 1942 – July 4, 1995) was an American painter, art instructor, and television host. He was the creator and host of ''The Joy of Painting'', an instructional television program that aired from 1983 to 1994 on PBS in the United States, CBC in Canada, and similar channels in Latin America, Europe and elsewhere. Ross would subsequently become widely known through his posthumous internet presence. Early life Ross was born in Daytona Beach, Florida, to Jack and Ollie Ross, a carpenter and a waitress respectively, and raised in Orlando, Florida. As an adolescent, Ross cared for injured animals, including armadillos, snakes, alligators and squirrels, one of which was later featured in several episodes of his television show. He had a half-brother, Jim, whom he mentioned in passing on his show. Ross dropped out of high school in the 9th grade. While working as a carpenter with his father, he lost part of his left index finger, which did not affec ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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